Molecular biology-based assays that detect the HCV RNA genome: Branched-chain DNA Although this method is easier to apply to a large number of samples, it is relatively insensitive. It measures HCV-RNA levels only above 200,000 viral particles per milliliter.
Nonspecific tests that measure liver enzymes: Before the introduction of PCR-based tests, biochemical testing was the standard method of testing for the presence of HCV infection. Nonspecific biochemical tests that measure the levels of liver enzymes such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are used to assess liver function. Raised enzyme levels are an indicator of liver disease.
